Coding

Part:BBa_K1964003:Design

Designed by: Steffen Lütke   Group: iGEM16_Marburg   (2016-10-14)


Codon optimized Cytochrome P450 for POH synthesis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1461


Design Notes

During the design the T2A-sequences for the correct cleavage of the peptide-sequences were added with slightly different DNA-sequences to avoid unwanted homologous recombination. Biobrick cutsites had to be removed from the codon optimized sequence for Saccharomyces cerevisiae before ordering the gene blocks from idtDNA.


Source

The genes were originally found in Mycobacterium sp. strain HXN-1500. The codon optimized genomic sequence used including the T2A-sequences have been synthesized via idtDNA.

References